Universiteit Leiden Opleiding Informatica

نویسنده

  • T. Vermeulen
چکیده

In neurons, the dendritic structure is sensitive to changes in the environment. Several treatments have been known to create changes in dendritic structure. 3D confocal images have been used before to evaluate and quantify changes in dendritic structure. In this research, specific treatment with a lentivirus in vivo lead to green fluorescent protein expression. Moreover, the lentiviruses were engineered to knock down the glucocorticoid receptor. Based on these biological features our work created a method for analysing dendritic spines in a semi-automated fashion, which involves the use of a confocal laser scanning microscope, deconvolution, the NeuronStudio software, statistical analysis using SPSS and the WEKA data mining tool. This research showed that the glucocorticoid receptor knockdown causes significant morphological changes in spine structures in the hippocampus of mouse brains. These changes indicate more spine maturation.

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تاریخ انتشار 2010